Οξεία Μυελογενής Λευχαιμία

• PML-RARa / t(15;17) (RT-PCR)*
• FLT3-ITD (PCR και Fragment Analysis)
• FLT3-TKD (PCR)
• NPM1 (PCR και Fragment Analysis)
• CEBPA (PCR και Sanger sequencing)
• AML-ETO / t(8;21) (RT-PCR)*
• INVERSION 3 / t(3;3) (RT-PCR)
• INVERSION 16 / t(16;16)(RT-PCR)*
• IDH1 (PCR και Sanger Sequencing)
• IDH2 (PCR και Sanger Sequencing)

• NEXT GENERATION SEQUENCING, Oncomine Myeloid panel DNA

Ανάλυση γονιδίων:
Hotspots genes: ABL1, BRAF, CBL, CSF3R, DNMT3A, FLT3, GATA2, HRAS, IDH1, IDH2, JAK2, KIT, KRAS, MPL, MYD88, NPM 1, NRAS, PTPN11, SETBP1, SF3B1, SRSF2, U2AF1, WT1
Full genes: ASXL1, BCOR, CALR, CEBPA, ETV6, EZH2, IKZF1, NF1, PHF6, PRPF8, RB1, RUNX1, SH2B3, STAG2, TET2, TP53, ZRSR2

• NEXT GENERATION SEQUENCING, Oncomine Myeloid panel RNA

Ανάλυση γονιδίων:
Fusion driver genes: ABL1, ALK, BCL2, BRAF, CCND1, CREBBP, EGFR, ETV6, FGFR1, FGFR2, FUS, HMGA2, JAK2, KMT2A (MLL), MECOM, MET, MLLT10, MLLT3, MYBL1, MYH11, NTRK3, NUP214, PDGFRA, PDGFRB, RARA, RBM15, RUNX1, TCF3, TFE3
Expression genes: BAALC, MECOM, MYC, SMC1A, WT1
Expression control genes: EIF2B1, FBXW2, PSMB2, PUM1, TRIM27